RNA

Part:BBa_K4950002:Design

Designed by: Lucas Ng   Group: iGEM23_PennState   (2023-10-12)


mCRP1 Riboswitch Insert


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Previously published apatamer sequence was required along with its known binding affinity [1]. Design was selected for its high predicted activation ratio and distinctness from other designs.

Source

De Novo DNA Riboswitch Calculator

References

[1] Bini, A., Centi, S., Tombelli, S., Minunni, M., & Mascini, M. (2008). Development of an optical RNA-based aptasensor for C-reactive protein. Analytical and bioanalytical chemistry, 390(4), 1077–1086. https://doi.org/10.1007/s00216-007-1736-7